Thymosin fragment (research)
TB-500
Lyophilized research peptide
Synthetic thymosin beta-4 fragment widely studied in vitro for actin-binding and migration assays. Lyophilized, ≥99% HPLC. Research use only.
Lab name and RUO acknowledgment are collected at checkout.
Lot documentation
Every shipment includes the lot-specific certificate of analysis — HPLC purity (≥99% typical), mass-spec identity, net peptide content, water content, and residual solvents.
Identity & specifications
- Common name
- TB-500 (Thymosin Beta-4 fragment)
- Sequence (Tβ4 17–23 motif)
- LKKTETQ — published synthetic analogue
- Molecular weight
- 4,963 g/mol (full Tβ4); fragment MW listed on CoA
- CAS
- 77591-33-4 (Tβ4 parent)
- Appearance
- White lyophilized powder
- Purity (HPLC)
- ≥ 99%
- Net peptide
- Listed on CoA
- Solubility
- Soluble in bacteriostatic or sterile water
TB-500 in laboratory research
TB-500 is a synthetic peptide corresponding to the active region of Thymosin Beta-4 (Tβ4). Tβ4 is a 43-residue G-actin sequestering protein extensively studied in vitro for its role in cytoskeletal dynamics, cell migration, and angiogenesis pathways across mammalian cell models. Synthetic TB-500 is used by labs as a sequence-defined surrogate for the active region in cell-culture and biochemical assays.
Identity and characterization
TB-500 ships with HPLC purity and mass-spectrometry identity data on the certificate of analysis. Labs running comparative work commonly pair TB-500 with a Tβ4-positive reference and a scrambled-sequence negative control; this listing is structured so your QA team can attach those references to the SKU in your procurement system.
Storage
Hold lyophilized vials at -20 °C protected from light. Reconstituted aliquots should be stored at 2-8 °C for short-term use or frozen in single-use working volumes. Do not subject reconstituted material to repeated freeze/thaw — peptide bond integrity is best preserved by aliquot planning before initial reconstitution.
Documentation
CoA, batch records, and a moisture/residuals summary where in scope. Documentation is filed by SKU and lot so procurement and EHS teams can retrieve records during audit.
Research guide
TB-500 research overview
TB-500 is listed as a research-use-only material for qualified laboratory programs. This page connects the product listing with identity fields, storage context, CoA review, and long-form research resources so procurement and QA reviewers can evaluate the SKU without relying on a thin commerce page.
The catalog classifies this material as Thymosin fragment (research), which helps labs compare it with adjacent research peptides, organize internal inventory, and build study-specific reference groups.
Key identity fields
- Common name
- TB-500 (Thymosin Beta-4 fragment)
- Sequence (Tβ4 17–23 motif)
- LKKTETQ — published synthetic analogue
- Molecular weight
- 4,963 g/mol (full Tβ4); fragment MW listed on CoA
- CAS
- 77591-33-4 (Tβ4 parent)
- Appearance
- White lyophilized powder
- Purity (HPLC)
- ≥ 99%
Storage and handling context
For long-tail stability searches, TB-500 should be evaluated in the context of lyophilized storage, reconstitution buffer choice, aliquot planning, freeze-thaw limits, and lot-specific CoA conditions. Always follow institutional SOPs and the documentation attached to the received lot.
Mechanism context
TB-500 should be described through published model-system language: receptor family, pathway context, assay controls, concentration planning, and lot traceability. Avoid transferring assumptions from adjacent peptides without a documented comparison protocol.
Search intent covered
- TB-500 storage and stability
- TB-500 CoA and identity
- TB-500 mechanism research
- TB-500 comparison resources
Product FAQ
Is TB-500 intended for clinical programs?
No. BluGen listings are research use only (RUO) materials for qualified laboratory programs, not clinical, diagnostic, therapeutic, veterinary, food, drug, or cosmetic use.
What documentation is available for TB-500?
Lot-level documentation may include CoA, HPLC purity, MS identity confirmation, net peptide content, and batch records where available for the SKU and lot.
How should lyophilized TB-500 be stored?
Store sealed lyophilized vials cold, dry, and protected from light unless the lot CoA or institutional SOP specifies another condition. Reconstituted material should be aliquoted according to the lab protocol.
Where can I learn more about TB-500 research context?
See the BluGen research guide for mechanism context, stability notes, storage considerations, and related peptide comparisons: https://getblugen.com/research/research-guide-tb-500/
What is the research category for TB-500?
TB-500 is categorized as Thymosin fragment (research) for catalog organization, internal linking, and related research comparisons.
What sequence is listed for TB-500?
Sequence identity should be verified against the lot-specific CoA and product identity table before release into a research workflow.
What molecular weight is listed for TB-500?
The listed molecular weight for TB-500 is 4,963 g/mol (full Tβ4); fragment MW listed on CoA. Use the lot CoA and net peptide content when calculating research stock concentrations.
Does TB-500 require cold storage?
Most lyophilized research peptides are stored cold, dry, sealed, and protected from light unless the lot CoA or institutional SOP states otherwise.
How should reconstituted TB-500 be handled?
Reconstituted research stocks should be labeled with SKU, lot, solvent, concentration, date, and technician initials, then aliquoted according to the lab protocol to limit freeze-thaw history.
Can lots of TB-500 be mixed together?
Avoid mixing lots unless a written protocol requires it. Mixed-lot stocks reduce traceability and make CoA review more difficult.
What purity documentation matters for TB-500?
HPLC purity, MS identity, net peptide content, moisture or residual summaries where available, and lot number alignment are the core documentation checks.
What does net peptide content mean for TB-500?
Net peptide content accounts for peptide mass apart from water and counter-ion contribution. It is important for molarity calculations in laboratory records.
What should procurement review before ordering TB-500?
Procurement should review SKU, vial size, lot documentation path, RUO status, storage requirements, shipping expectations, and any internal approval requirements.
How should TB-500 be cited in a method record?
Record supplier, SKU, lot number, CoA document ID, reconstitution solvent, stock concentration, storage condition, and date prepared.
What are common related resources for TB-500?
Related resources include the peptide storage guide, reconstitution guide, CoA reading guide, purity guide, and any comparison articles linked from the product page.
Why does BluGen link TB-500 to research articles?
Internal article links help buyers evaluate identity, storage, documentation, and research context without relying on a thin product listing.
What should be done if TB-500 documentation is incomplete?
Hold the lot in review and request the missing CoA or batch documentation before releasing the material into an internal research workflow.
Is TB-500 content on BluGen medical advice?
No. BluGen content is laboratory procurement and research documentation content for RUO materials only.
Related research resources
Reference path
- Lot CoA: verify HPLC purity, MS identity, and net peptide before release.
- Internal SOP: record storage, reconstitution, concentration, and aliquot history.
- Reconstitution calculator: convert vial size + diluent to concentration
- Article links: file the product guide, glossary definition, storage guide, and comparison page beside procurement notes.
- Search PubMed for TB-500 research
Research use only (RUO)
This material is sold for laboratory research purposes only. It is not for clinical, diagnostic, therapeutic, or veterinary application, and not for food, drug, or cosmetic use where restricted.