TB-500: Research overview, identity, and laboratory handling guide

8 min read · Research use only

Written and reviewed by BluGen Research Team · Editorial standards

Synthetic thymosin beta-4 fragment widely studied in vitro for actin-binding and migration assays. Lyophilized, ≥99% HPLC. Research use only. This guide covers identity, published research context, procurement checks, and storage — RUO only, no clinical claims.

What TB-500 is in published research

TB-500 is a synthetic peptide corresponding to the active region of Thymosin Beta-4 (Tβ4). Tβ4 is a 43-residue G-actin sequestering protein extensively studied in vitro for its role in cytoskeletal dynamics, cell migration, and angiogenesis pathways across mammalian cell models. Synthetic TB-500 is used by labs as a sequence-defined surrogate for the active region in cell-culture and biochemical assays.

TB-500 ships with HPLC purity and mass-spectrometry identity data on the certificate of analysis. Labs running comparative work commonly pair TB-500 with a Tβ4-positive reference and a scrambled-sequence negative control; this listing exposes structured identity fields so procurement systems can index the SKU against those reference standards.

Hold lyophilized vials at -20 °C protected from light. Reconstituted aliquots should be stored at 2-8 °C for short-term use or frozen in single-use working volumes. Do not subject reconstituted material to repeated freeze/thaw — peptide bond integrity is best preserved by aliquot planning before initial reconstitution.

CoA, batch records, and a moisture/residuals summary where in scope. Documentation is filed by SKU and lot so procurement and EHS teams can retrieve records during audit.

The material class "Thymosin fragment (research)" helps procurement teams group TB-500 with related reference peptides during comparative studies.

Sequence and identity

TB-500 identity on the catalog: Common name: TB-500 (Thymosin Beta-4 fragment); Sequence (Tβ4 17–23 motif): LKKTETQ — published synthetic analogue; Molecular weight: 4,963 g/mol (full Tβ4); fragment MW listed on CoA; CAS: 77591-33-4 (Tβ4 parent); Appearance: White lyophilized powder; Purity (HPLC): ≥ 99%; Net peptide: Listed on CoA; Solubility: Soluble in bacteriostatic or sterile water. Copy these into receiving and LIMS before reconstitution.

Mechanisms studied in published research

Mechanism-focused research on TB-500 is typically conducted in cell lines or biochemical preparations that express the relevant receptor or binding protein for its class.

Design controls that include scrambled-sequence or orthogonal-receptor negative controls when your protocol evaluates TB-500 pharmacology in vitro.

Report effect sizes with lot number and CoA reference so results can be reproduced if the same TB-500 SKU is reordered later.

Avoid extrapolating in vitro binding or signaling readouts to whole-organism outcomes; RUO materials are not qualified for clinical investigation.

Procurement and identity verification

Request the lot-specific CoA for TB-500 at receipt. Verify SKU, variant size if applicable, and identity mass before the lot enters general storage.

Compare HPLC purity to your internal minimum for the peptide family. File chromatogram excerpts when your QMS requires raw data.

Enter net peptide content from the CoA into inventory before calculating molarity for TB-500 working stocks.

Attach purchase order, packing list, and CoA in one audit folder per lot.

Storage and reconstitution

Store lyophilized TB-500 sealed at -20 °C protected from light unless the CoA states otherwise.

Reconstitute with bacteriostatic water or a buffer validated for your assay pH. Aliquot to limit freeze-thaw.

For oxidation-sensitive sequences in TB-500, minimize open-vial time at room temperature during weighing.

See our lyophilized storage guide for institution-scale SOP examples and aliquot labeling conventions.

Comparative reference points

Researchers comparing TB-500 with adjacent catalog references often evaluate the following SKUs in parallel plates:

Use matched reconstitution buffers and stock concentrations when running comparative binding or signaling assays.

Document reviewers should cross-link this guide with the product certificate of analysis and internal receiving SOP.

When publishing methods, cite lot number, SKU, reconstitution buffer, and stock concentration so external labs can interpret your figures.

Institutional procurement may require RUO acknowledgment at checkout; store that acknowledgment beside batch records for audits.